Expression, purification, crystallization and preliminary crystallographic analysis of the human intracellular chloride channel protein CLIC4.

نویسندگان

  • De-Feng Li
  • Yun-Feng Li
  • Qiu-Hua Huang
  • Ying Zhang
  • Da-Cheng Wang
چکیده

The human chloride intracellular channel protein CLIC4 has been crystallized by the hanging-drop vapour-diffusion technique using trisodium citrate as the precipitant. The best crystals were obtained by the microseeding method. The crystals diffracted to 2.2 A resolution and were found to belong to space group P121, with unit-cell parameters a = 73.19, b =86.05, c = 73.38 A, beta = 112.99 degrees and three molecule per asymmetric unit.

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عنوان ژورنال:
  • Protein and peptide letters

دوره 13 6  شماره 

صفحات  -

تاریخ انتشار 2006